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anti-tubulin βiii mouse monoclonal sc-80005  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti-tubulin βiii mouse monoclonal sc-80005
    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin <t>βIII–immunostaining</t> for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).
    Anti Tubulin βiii Mouse Monoclonal Sc 80005, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-tubulin βiii mouse monoclonal sc-80005/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    anti-tubulin βiii mouse monoclonal sc-80005 - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats"

    Article Title: Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2025.1619310

    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).
    Figure Legend Snippet: Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).

    Techniques Used: Light Microscopy, Staining, Immunostaining

    Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).
    Figure Legend Snippet: Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).

    Techniques Used:



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    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin <t>βIII–immunostaining</t> for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).
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    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after <t>tubulin</t> <t>βIII–immunostaining</t> for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).
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    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after <t>tubulin</t> <t>βIII–immunostaining</t> for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).
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    Image Search Results


    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats

    doi: 10.3389/fncel.2025.1619310

    Figure Lengend Snippet: Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).

    Article Snippet: Thereafter, the specimens were incubated with anti-tubulin βIII mouse monoclonal (sc-80005; Santa Cruz Biotechnology) and anti-CGRP rabbit polyclonal (Y340; Yanaihara Institute Inc., Shizuoka, Japan) as primary antibodies (both diluted 1:200), for 2 days at 4°C.

    Techniques: Light Microscopy, Staining, Immunostaining

    Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats

    doi: 10.3389/fncel.2025.1619310

    Figure Lengend Snippet: Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).

    Article Snippet: Thereafter, the specimens were incubated with anti-tubulin βIII mouse monoclonal (sc-80005; Santa Cruz Biotechnology) and anti-CGRP rabbit polyclonal (Y340; Yanaihara Institute Inc., Shizuoka, Japan) as primary antibodies (both diluted 1:200), for 2 days at 4°C.

    Techniques:

    Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats

    doi: 10.3389/fncel.2025.1619310

    Figure Lengend Snippet: Histological alterations in the cornea at different time points after lacrimal gland excision. (A) Light microscopy images of the central cornea after hematoxylin and eosin staining for the sham side at 1 week post-surgery and the excision side at 1, 4 and 8 week post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). The arrow indicates the neovascular formations in the stroma. (B) Quantitative analysis of the number of cell nuclei in the stroma. Results are presented as the mean ± SEM ( n = 5 for each group. Statistical significance was assessed using two-way ANOVA, * P < 0.05 and ** P < 0.01, versus the sham side; ## P < 0.01 versus 1-week post-surgery). (C) Light microscopy images of the central cornea after tubulin βIII–immunostaining for the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery (Left panel: original magnification, × 40; scale bar, 50 μm. Right panel: magnified images within the dashed line in the left panel.). (D) Quantitative analysis of the tubulin βIII-positive area in the epithelium, representing nerve density. Each column and the vertical bar represent the mean ± SEM ( n = 5 for each group; Two-way ANOVA, * P < 0.05 compared to the sham side, and ## P < 0.01 compared to the 1-week post-surgery).

    Article Snippet: For immunohistochemical staining, the sections were incubated for overnight at 4°C with an anti-tubulin βIII mouse monoclonal antibody (sc-80005; Santa Cruz Biotechnology, Dallas, TX, USA) diluted 1:200, an anti-Sema3A mouse monoclonal antibody (sc-74554, diluted 1:1000; Santa Cruz Biotechnology) and an anti-Sema7A mouse monoclonal antibody (sc-374432, diluted 1:400; Santa Cruz Biotechnology).

    Techniques: Light Microscopy, Staining, Immunostaining

    Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Tear deficiency transforms spatial distribution of corneal calcitonin gene-related peptide-positive nerves in rats

    doi: 10.3389/fncel.2025.1619310

    Figure Lengend Snippet: Changes in corneal nerve morphology in whole-mount preparations after lacrimal gland excision. The localization of tubulin βIII–positive nerve fibers (red) and CGRP–positive nerve fibers (green) in the sham side at 1 week post-surgery and the excision side at 1, 4, and 8 weeks post-surgery. (A) Corneal subbasal nerve plexus in the epithelium (original magnification, × 20; scale bar, 50 μm). (B) Corneal nerve innervation in the stroma at a depth of approximately 50–100 μm from the corneal surface (original magnification, × 20; scale bar, 50 μm).

    Article Snippet: For immunohistochemical staining, the sections were incubated for overnight at 4°C with an anti-tubulin βIII mouse monoclonal antibody (sc-80005; Santa Cruz Biotechnology, Dallas, TX, USA) diluted 1:200, an anti-Sema3A mouse monoclonal antibody (sc-74554, diluted 1:1000; Santa Cruz Biotechnology) and an anti-Sema7A mouse monoclonal antibody (sc-374432, diluted 1:400; Santa Cruz Biotechnology).

    Techniques: